Thermo Ion Torrent Personal Genome Machine, including the Ion OneTouch 2 System, DNA Sequencing
Thermo Ion Torrent Personal Genome Machine, including the Ion OneTouch 2 System
With Data Collection Network PC
Ion Torrent’s Personal Genome Machine is a semiconductor chip that holds 1.5 million sensors, each of which can hold a single strand DNA fragment. The chip electronically detects the DNA sequence, unlike other sequencing machines that optically detect DNA with pricey lasers, microscopes, and cameras. It can sequence a DNA sample in a few hours, while other machines can take at least a week. And it can scale up fast.
The Ion Torrent™ Systems combine semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data.
The Chip is the Machine™ and Ion Torrent™ directly benefits from four decades of exponential improvement in semiconductor technology. This enables the chips to scale in density for any research application—from small, targeted experiments to large genomes—all on a single platform. See how Ion Torrent Chips work.
With as little as 10 ng input DNA, the new Ion 314™, 316™, and 318™ Chips yield 99.99% consensus accuracy and up to a 50% increase in output over previous chips, in as little as 2 hours on the Ion PGM™ System.
For whole genome sequencing on the Ion Proton™ System, the Ion PI™ Chip enables the highest throughput with up to 10 Gb reads in 2–4 hours.
This unique combination of simplicity and easy scalability makes semiconductor sequencing an accessible next-generation sequencing solution for all researchers, from whole genome/transcriptome to small genome and small RNA sequencing applications.
Ion PGM™ Chip Run time Output
200 bp read 400 bp read 200 bp read 400 bp read
Ion 314™ Chip v2 2.3 hr 3.7 hr 30–50 Mb 60–100 Mb
Ion 316™ Chip v2 3.0 hr 4.9 hr 300–500 Mb 600 Mb–1 Gb
Ion 318™ Chip v2 4.4 hr 7.3 hr 600 Mb–1 Gb 1.2–2 Gb
Ion Proton™ Chip Run time Output
200 bp read 200 bp read
Ion PI™ Chip 2–4 hr Up to 10 Gb
Ion Torrent One Touch: (thermo catalog no 4474779)
The Ion OneTouch 2 System performs template amplification and enrichment as part of the manual workflow for the Ion PGM, Ion Proton, and Ion S5 and Ion S5 XL systems. It provides scalable template preparation for all Ion semiconductor chips. With its small footprint, the system fits easily on any bench top.
For a fully automated workflow, please see the Ion Chef Instrument.
Efficiency and flexibility
The Ion OneTouch 2 System integrates multiple manual template preparation steps into a single system. This enables parallel processing of multiple samples per day through modular design. It is flexible and supports scalable template preparation for all Ion semiconductor sequencing chips with up to 400 bases on the Ion 520/530 chips, up to 200 bases on the Ion 540 chip, up to 400 bases on the Ion PGM System, and up to 200 bases on the Ion Proton System. The need for gas is eliminated by the use of a peristaltic pump.
The Ion OneTouch 2 System consists of two modules: the Ion OneTouch 2 Instrument and the Ion OneTouch ES (Enrichment System). The Ion OneTouch 2 Instrument may be purchased separately.
At the heart of the Ion OneTouch 2 Instrument are three breakthrough technologies that enable automated delivery of the template Ion Sphere particles. The first is the reaction filter that creates millions of microreactors in which clonal amplification occurs. The second is the fully integrated thermal cycler and disposable path amplification plate that enables robust thermal cycling of the microreactors. The third is the integrated centrifuge, which recovers the template Ion Sphere particles. Altogether this allows the instrument to deliver massively parallel clonal amplification and recovery, all in a small bench top footprint.
Designed to fit in any lab
With the included Ion OneTouch ES module, the Ion OneTouch 2 System enables parallel template processing with sample sequencing on the Ion PGM, Ion Proton, Ion S5, and Ion S5 XL systems. This reduces the time from sample to sequence to a single day and enables processing of multiple samples per day. The combination of Ion semiconductor sequencing and the Ion OneTouch 2 System maximizes the speed of each experiment and minimizes the overall time to completion for each project. The modular design also allows for QC of the Ion Sphere particles, to maximize experimental control and the success of each experiment.
Chip Compatibility: Ion 314™ Chip, Ion 316™ Chip, Ion 318™ Chip, Ion PI™ Chip
Dimensions: Ion OneTouch™ 2 Instrument: 12 x 16 x 14 in (30 x 41 x 36 cm), Ion OneTouch™ ES: 9.5 x 12.5 x 11 in (24 x32 x 28 cm)
For Use With (Equipment): Ion OneTouch™ 2 System, Ion PGM™ System, Ion Proton™ System
High Throughput Compatibility: Automated Protocols, High Throughput-Compatible, Multiplexing
Library Type: Fragment Library, Mate-Paired Library, Targeted Sequencing Library, cDNA Library
Multiplex Capability: Up to 384 barcoded libraries
Operating Environment: Humidity: 20%-80% (non-condensing), Temperature: 15°C-25°C
Product Line: Ion OneTouch™
Product Size: 1 system
Run Time: Hands-on time: Minutes, Ion PGM™ System: 5 hours total for template preparation, Ion Proton™ System: 8 hours total for template preparation
Voltage Limit: International: 220 V, US: 110 V
Weight: Ion OneTouch™ 2 Instrument: 23 lb (10.4 kg), Ion OneTouch™ ES: 12 lbs (5.4 kg)