Roche LightCycler 2.0 LC Centrifuge and Carousel
Roche LightCycler 2.0 LC Centrifuge and Carousel - Full system
With LightCycle 2.0 User Manual and LC Software 4 for 2.0 Manual
Software needs to be purchased seperately
Use the LightCycler® 2.0 Instrument to perform:
- Fast and sensitive real-time PCR and RT-PCR
- Reproducible and reliable kinetic quantification
- of DNA, cDNA, or RNA
- Easy PCR product identification and characterization
- by melting point analysis
- Highly specific mutation analysis and
- SNP (Single Nucleotide Polymorphism) genotyping
The LightCycler® 2.0 Instrument allows you to monitor
amplification of 32 PCR Products simultaneously, in
real-time and online, with six different detection channels.
The LightCycler® Software 4.1 collects fluorescence data
from all six channels at each cycle and displays it immediately.
This enables you to terminate PCR runs early if signal
levels are adequate, or prolong them when signals are too low.
Amplification occurs in specially designed reaction capillaries.
Their superior surface-to-volume ratio provides extremely
rapid thermal transfer, which facilitates high-speed
thermal cycling. The combination of LightCycler® Capillaries
and the air-driven temperature control of the LightCycler® 2.0
Instrument ensures rapid PCR. An entire 35-cycle run
can be performed in as little as 30 minutes (with 20 μl capillaries)
Fluorescence detection formats: The LightCycler® 2.0 Instrument
is optimized for two fluorescence detection formats: SYBR Green I,
HybProbe probes, and hydrolysis probes. In addition, the
instrument supports a wide variety of other fluorescence detection
formats, such as monocolor SimpleProbe probes and other formats
based on FRET (fluorescence resonance energy transfer). However,
it is essential that the fluorescent dyes used are compatible with the
The LightCycler® 2.0 optical unit.
Roche LightCycler 2.0 LC Centrifuge and Carousel
LC Centrifuge Application
The LC Carousel Centrifuge 2.0 is used to centrifuge PCR or RT-PCR samples in LightCycler® Capillaries. After the capillaries are placed into the LightCycler® 2.0 Sample Carousel, the reaction mix is pipetted into the polypropylene cups of the capillaries, manually or automatically, using the MagNA Pure LC Instrument. The capillaries are sealed with stoppers, and the LightCycler® 2.0 Sample Carousel is placed within the bucket into the centrifuge. After a preset centrifugation of 30 seconds, the LightCycler® 2.0 Sample Carousel can be inserted into the LightCycler® 2.0 Instrument for sample amplification.
The LC Carousel Centrifuge 2.0 is an easy-to-use centrifuge with a specially designed rotor that can hold one LightCycler® 2.0 Sample Carousel (for the 20 µl or 100 µl capillaries) of the LightCycler® 2.0 Instrument. - See more at:
Basel, 2 April 2009
Roche to introduce new LightCycler MRSA Advanced Test in the EU
New test for detection of methicillin-resistant Staphylococcus aureus for better patient care
Roche announced today that its new LightCycler test for the detection of methicillin-resistant Staphylococcus aureus (MRSA) is now available with the CE Mark, allowing it to be sold for clinical use in the European Union. The LightCycler MRSA Advanced Test is a qualitative in vitro diagnostic test for the direct detection of nasal colonization with methicillin-resistant Staphylococcus aureus. The test will aid in the prevention and control of MRSA infections in healthcare settings resulting in better patient care.
"As more guidelines recommend or require aggressive screening, it is important that healthcare professionals have access to fast, reliable products that can help improve medical outcomes,” said Daniel O’Day Head of Roche Molecular Diagnostics. “This new test will aid infection control programs, and ultimately result in better patient care.”
Healthcare-associated infections (HAIs) caused by MRSA have recently become an important issue for healthcare facilities worldwide due to high rates of infection, mortality, and high costs of treatment. In addition, community-associated MRSA (CA-MRSA) has spread in the past few years, feeding the pipeline of infection in hospitals, and underscoring the need for comprehensive infection control programs.
Dr. Reinhard Frodl, Laboratory Head of the Molecular Biology Department of Medizinisches Versorgungszentrum Dr. Gärtner & Kollegen in Ravensburg, Germany commented: “The test showed good sensitivity and specificity in our hands and we believe it will bring more efficient MRSA screening to hospitals across Europe because of its batching flexibility and easy handling.”
The LightCycler MRSA Advanced Test is performed on Roche’s LightCycler 2.0 Instrument with nasal swab specimens from patients suspected of colonization, using Roche’s patented real-time polymerase chain reaction (PCR) technology. Provided in a convenient, ready-to-use format, and designed for flexible batch sizes, the LightCycler MRSA Advanced Test ensures safety and productivity of laboratory staff, flexible throughput, and accurate and reliable results.
About methicillin-resistant Staphylococcus aureus (MRSA)
Staphylococcus aureus is responsible for many serious infections and is one of the most frequently isolated bacteria from patients with healthcare-associated infections (HAI). Estimates suggest that 4 million HAIs and 37,000 deaths are attributable to these infections each year in the EU, and that 1 out of 10 patients in an EU hospital acquires an HAI. Antimicrobial resistance and HAIs, either combined or separately, constitute a major infectious disease problem in the EU, and show signs of becoming more prevalent in the future.1 MRSA infections are a tremendous burden for healthcare systems and hospitals and are associated with significant healthcare costs. In 2004, the United Kingdom National Audit Office estimated that infections such as MRSA kill 5,000 people each year in the UK and hospital-associated infections cost the National Health Service around £1 billion a year.2
About the LightCycler and LC centrifuge System
With more than 6,600 LightCycler® Instruments in the market today, the LightCycler® System is among the most widely utilized real-time amplification systems available. The LightCycler® 2.0 Instrument was the first system to introduce hybridization probes, true melting curve analysis, automated absolute quantification, and relative quantification with efficiency correction. Combining the power of the LightCycler 2.0 Instrument with the simple, flexible, and reliable design of the LightCycler MRSA Advanced Test provides a powerful tool to support healthcare institutions in their fight against MRSA.
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